1a. ACI: Chromatography \ Mass Spectrometry: ACI: LC-MS/MS, Q-Exactive Orbitrap

What is it?
Liquid chromatography is a technique in analytical chemistry is used to separate individual components in a mixture with identification and quantification of each component through the Orbitrap Mass Spectrometry. Orbitrap is an ion trap mass analyzer consisting of an outer barrel-like electrode and a coaxial inner spindle-like electrode that traps ions in an orbital motion around the spindle.

Manufacturer: Thermo Scientific

Model Name: Q-Exactive Orbitrap

Applications: Analysis of both targeted and non-targeted analytes compounds.

Instrument Overview: HCD cell allows ions pass from the nitrogen-filled C-trap into this multipole collision cell and undergo fragmentation. Afterwards, HCD voltage is increased, and the ions are transferred back to the C-trap, to be injected into the Orbitrap for detection. Quadrupole Orbitrap mass spectrometers enable both targeted and non-targeted screening and characterization. High-resolution, accurate-mass (HRAM) performance advantages offered by Orbitrap mass analyzers connect to liquid chromatography equipment to enhance separation of unknown and known compounds and enable high-throughput workflows

Technical features and specifications:
Sample Type: Liquid/ Solid
Mass Range: 50 to 6000m/z
Mass Accuracy: <1ppm
Mass Resolution: > 140000
Scan Speed: 12GHz
Ion Source: ESI/APCI

Charges: NA

Instrument Manufacturer URL:
https://www.thermofisher.com/sg/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms.html

Detailed Specifications: NA