BD FACSymphony A3 (Alto)
What is it?
A 5-laser, multi-parameter cellular analyser with 28 fluorescent detectors plus 2 scatter parameters.
Manufacturer: Becton Dickinson
Model Name: BD FACSymphony A3
Applications: Multiplex Cell Analysis
Instrument Overview: Multi-laser, multi-parameter analysis cytometer with 5 lasers (Blue, Yellow-green, Violet, Red and UV) that allow detection of up to 28 colors simultaneously. This distribution of fluorochromes over five lasers reduces spectral overlap and improves sensitivity, yielding more information from each sample.
Technical features and specifications:
Software: BD FACSDiva
Sample: Single tube, 96-well plate
Lasers: Blue (488nm), Yellow-Green (561nm), Violet (405nm), Red (628nm) and UV (349nm)
Detector channel representative fluorophores:
Blue: FITC/GFP, BB630, BB660, PerCP-Cy5.5, BB790
Yellow-Green: PE/RFP, PE-TexasRed/PI/mCherry, PE-Cy5, PE-Cy7
Violet: BV421, BV480, BV510, BV570, BV605, BV650, BV711, BV750, BV786
Red: APC, AlexaFluor700, APC-Cy7
UV: BUV395, DAPI, BUV563, BUV615, BUV661, BUV737, BUV805
For the full instrument configuration and filter sets, please email SIgN Flow Cytometry Platform.
Instrument Manufacturer URL: Becton Dickinson
Detailed Specifications: BD FACSymphony A3
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