What is it?
Adaptable optics, with many filter, laser and configuration options, and the unique fluidics system provide flexibility in the fluorochrome combinations you can analyze and automation for direct counting of cells.
Manufacturer: BD Biosciences
Model Name: BD FACSymphony™ A5 SE Cell Analyzer
Applications: Flow Cytometer
Instrument Overview: Fluorescence sensitivity is dependent on selected configuration, laser wavelengths, laser power, PMTs and filters. Molecules of equivalent soluble fluorochrome (MESF) measurements, using SPHERO™ Rainbow Calibration Particles (RCP-30-5A), were performed on multiple instruments with 355-nm (60 mW), 405- nm (200 mW), 488-nm (150 mW), 637-nm (140 mW) and 561- nm (150 mW) lasers.
Each sensor is automatically recorded during acquisition, providing quality control for imaging experiments and enhancing data reproducibility.
Technical features and specifications:
The BD FACSymphony™ A5 SE Cell Analyzer comes with five standard lasers: 355 nm - 60 mW 405 nm - 200 mW 488 nm - 150 mW 561 nm - 150 mW 637 nm - 140 mW
Rectangular quartz cuvette: Internal cross-section, 430 x 180 μm
UV (355 nm): 10 detectors between 365 nm–850 nm Violet (405 nm): 14 detectors between 415 nm–880 nm Blue (488 nm): 9 detectors between 500 nm–849.5 nm Yellow-Green (561 nm): 9 detectors between 570 nm–850 nm Red (637 nm): 6 detectors between 645 nm–810 nm
Software: BD FACSDiva™ Software v9.3 (Documentation) Software
Charges: S$100 per hour
Instrument Manufacturer URL: NA
Detailed Specifications:
Equipment URL:
https://www.bdbiosciences.com/en-sg/products/instruments/flow-cytometers/research-cell-analyzers/bd-facsymphony-a5-seOther Equipment from A*STAR IDL
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