A*STAR Scientific Equipment Finder

Colony Picker: Molecular Devices QPix HT w/ Auto Cloning Platform

equipment

What is it?
Automation platform for performing high-throughput molecular biology workflows such as cloning, enzyme assay and cell evolution etc.

Manufacturer(s): Molecular Devices, Beckman Coulter, 4titude, Brooks, Liconic, Bionex, Inheco

Model Name: QPix 420 (Colony Picker), Biomek i7 (Liquid Handler), a4s (Plate Sealer), Xpeel (Plate De-sealer), LPX110/STX44 (Storage/Incubator Units), HiG4 (Ambient Centrifuge), ODTC96, ThermoShake, CPAC (Thermal Cycler, Temp Control Shaker and Static Temp Control), iD3 (Microplate reader)

Applications: Genomics (DNA Cloning and Transformation, Plasmid/DNA Extraction), Cell Biology (Cell Line Development, Multipart Assays) and Proteomics (Protein Production and Extraction)

Instrument Overview: Integrated platform to automate labour intensive workflow, able to perform customized experiments with high precision.

Technical features and specifications:

QPix: Colony Picking, Fluorescent or Blue-White Screening and Picking, Plating and Plate Replication. Picking of colonies into 96-well Standard and Deep Well Plates, from 90mm Petri Dish, of Nunc Omni Trays (8-Well or Single-Well).
Wavelength Filters:
Ex /Em: 377 /447 nm for DAPI /Hoechst
Ex /Em: 457 /536 nm for FITC /GFP
Ex /Em: 531 /593 nm for Cy3 /DS Red
Ex /Em: 628 /692 nm for Cy5
Ex /Em: 531 /624 nm for Rhodamine /Texas Red

Biomek i7: 45 deck positions, 1 - 300ul 96-Channel Head, 500ul Span-8 Pod, Temperature Control on CPAC (4 - 50C), Shaking and Temperature Control on ThermoShake (0 – 1500 rpm, 25 – 50C)

LPX110: Max. 21 Deep Well (45mm) Storage and 44 Microtiter Plate (17mm) Storage

STX44: Max. 44 Deep Well Storage, Temperature Control (33 - 50C), 0 - 600 rpm Shaking

HiG4 Centrifuge: Up to 4000Gs, Plate-format only (w or w/o filter)

iD3 Spectrometer: Absorbance, Fluorescence and Luminescence plate reader. Compatible with 96-Well and 384-Well Plates.
Read Types
Endpoint, Kinetic, Spectrum Scan, Well Scan
Read Modes and Wavelength
Abs: 230 - 1000 nm
FL Ex: 250 - 830 nm
FL Em: 270 - 850 nm
Lumi: 300 - 850 nm

Instrument Manufacturer URL:
NA

Detailed Specifications:
QPix420
Biomek i7
a4s Plate Sealer
Xpeel De-sealer
LPX110
STX44
HiG4
ODTC96
ThermoShake
CPAC
iD3 Spectrometer

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