A*STAR Scientific Equipment Finder

Confocal: Zeiss LSM800 Inverted with Env Chamber

equipment

61 Biopolis Drive #06-20 Proteos Singapore 138673

What is it?
A highly motorised confocal laser scanning microscope for multidimensional acquisition (xyzt in multiple channels, tiles, bleaching) of live samples.

Manufacturer: Carl Zeiss

Model Name: LSM800 scan-head sits on Axio Observer.Z1 main body

Applications: Confocal microscopy, 2D and 3D Live-cell imaging, time lapse, bleaching

Instrument Overview: The laser scan confocal is based on an inverted Observer.Z1 microscope, and has all the standard multi-dimensional, multi-position and tiling imaging over time for live samples. In addition, confocal imaging would benefit the most from increased sensitivity, resolution, and speed from 2 sensitive GaAsP detectors , with up to 8 frames/sec with 512 x 512 pixels and up to 6144 x 6144 pixels resolution. The tiling function in the Zen Blue software is quite powerful and allows one to collect multiple images across a large field of view and/or pre-defined positions in multi-well plates using both low and high magnification objectives. In addition, the dedicated experiment-designer module allows complicated imaging process planning.

Technical features and specifications:
Objective lens: 10x, 20x, 40x (water), 63x (water), 63x (oil), 100x (oil)
Filters: DAPI, eGFP, TRITC
Light sources: Transmitted (HAL100), Fluorescence (HXP 120)
Laser lines: 405nm, 488nm, 561nm, 640nm
Environmental Control: PeCon XL Multi S1 DARK LS: heating Unit XL S, CO2 Module S, Temp Module S
Detectors: 2 GaAsP
Software: Zen blue 2.3

Charges:
To be updated

Equipment URL:

https://docs.google.com/spreadsheets/d/1T_t_jx9u4VlRQoe6AtewmwgbpJ2UWGyIyRI8kkn6Xes/edit#gid=1597193779

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