Imaging Systems: (EARO-HTP) PerkinElmer Operetta CLS (High-Content)

What is it?
An LED light source, fully automated confocal microscope meant for high-content screening. The system delivers speed, sensitivity and resolution to capture fine sub-cellular details and lets you find subtle phenotypic changes for simple and complex cellular feature extraction and quantification.

Manufacturer: PerkinElmer

Model Name: Operetta CLS

Applications: High-Content Screening/Imaging; Phenotypic Screening; Fluorescence & Brightfield Microscopy

Instrument Overview: The instrument can only accept plates, typically, 96- and 384-well plates. It has eight LEDs for a wide excitation range paired with confocal and widefield fluorescence. Its large format sCMOS camera delivers low noise, wide dynamic range, and high resolution. It also has a live-cell chamber with CO2 & temperature control and one water-immersion & three air objectives.

Technical features and specifications:
a. Compatibility: Microplates – imaging-qualified clear-bottom 96-/384-well plates
b. Automation: PlateHandler II Robot for continuous loading up to 28 microplates
c. 4 Objective lens: 1. 5x Air, NA 0.16
2. 10x Air, NA 0.3
3. 20x Air; NA 0.4
4. 20x Water, NA 1.0

d. 8 Excitation LEDs [nm]: Wavelength [nm]
1. 355-385,
2. 390-420,
3. 435-460,
4. 460-490,
5. 490-515,
6. 530-560,
7. 615-645,
8. 650-675


e. 8 Emission Filters [nm]: Wavelength [nm]
1. 430-500,
2. 470-515,
3. 500-550,
4. 525-580,
5. 570-620,
6. 570-650,
7. 655-705,
8. 655-760)

f. Light sources: Transmitted (LED), Fluorescence (LED)
g. Imaging Modes: Confocal & Widefield
h. Camera: Single large format sCMOS
i. Stage: Fully automated
j. Live Cell Chamber: Yes
k. Software: Harmony (Acquisition & Analysis), Columbus (Storage & Analysis)

Usage Cost:
• Please email us at earo@eddc.sg

Instrument Manufacturer URL: https://www.perkinelmer.com/product/operetta-cls-system-hh16000000

Detailed Specifications:
Please send your enquiry for more details.