What is it?
Agilent Seahorse XF Pro analyzer measures and reports the oxygen consumption rate (OCR), proton efflux rate (PER) or extracellular acidification rate (ECAR), as well as ATP production rates of live cells in a 96-well format. This analyzer features excellent OCR precision at low rates, verified performance, optimized temperature control, and is automation enabled. OCR, PER, and ATP production rates are critical measurements of energy metabolism and vital indicators of mitochondrial health, toxicity, glycolysis, and overall cellular (dys)function. Together, these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.
Manufacturer: Agilent
Model Name: Seahorse XF Pro
Applications: The Seahorse XF Analyzer is widely used to profile cellular bioenergetics by measuring real-time oxygen consumption and extracellular acidification, enabling detailed assessment of mitochondrial respiration and glycolytic activity. In cellular metabolism studies, it provides insights into energy production, metabolic flexibility, and stress responses under different physiological or perturbation conditions. In immune metabolism, the Seahorse platform is instrumental in characterising metabolic reprogramming during immune cell activation, differentiation, and effector functions, including studies of T cells, NK cells, and macrophages. In cancer metabolism, it supports investigation of altered metabolic phenotypes such as the Warburg effect, mitochondrial dysfunction, and metabolic vulnerabilities, as well as evaluation of responses to metabolic inhibitors and immuno-oncology strategies. Beyond biomedical research, the Seahorse XF Analyzer is increasingly applied in cultured meat research to assess metabolic efficiency, mitochondrial health, and nutrient utilisation of muscle and fat cells, supporting optimisation of cell growth conditions, media formulations, and scalability for sustainable food production.
Instrument Overview: The XF Pro analyzer is also equipped with advanced software, standardized workflows, and advanced data analytics available in the Agilent Seahorse Analytics software.
Technical features and specifications:
Live cell, real-time analysis of cellular energy metabolism platform in 96-well format
Multiple parameters reported from each assay well, including OCR, PER or ECAR, and ATP product rates
Excellent OCR precision at low rates, providing confidence when working with quiescent cell types, such as naïve T cells
High-sensitivity analysis of as few as 5,000 cells per well using the custom 96-well plate
Compatible with XF Pro M plate to reduce edge effect
Four-port injection system with automated mixing feature for assessing immediate cellular responses to substrates, inhibitors, and other compounds in real time
Verified instrument performance to ensure intraplate and interplate data consistency
Wide operation environment temperature of 4 to 30 °C, allowing the analyzer to maintain internal assay temperature of 16 to 42 °C
Advanced Pro Controller software for easy assay protocol creation with user guided interface
Data Quality View in Wave Pro software for automated flagging of outliers
Charges:
Depending on project requirement
Instrument Manufacturer URL: https://www.agilent.com/en/product/cell-analysis/real-time-cell-metabolic-analysis/xf-analyzers/seahorse-xf-pro-analyzer-1980223
Detailed Specifications:
NA
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