Lightsheet: Ultramicroscope II

What it is:
A light sheet fluorescence microscope that enables you to visualize whole biological systems. It is a second generation selective plane illumination microscope for 3-dimensional imaging.

Manufacturer: Miltenyi BioTec

Model Name: UltraMicroscope II

Applications: Light sheet fluorescence microscopy, Light sheet imaging

Instrument Overview: The UltraMicroscope II allows in vivo imaging as well as the imaging of cleared samples. All optical and mechanical components are compatible to most clearing solutions [DBE, Clarity,etc] and water. It also has a dual-side triple-sheet optics for generating two sets of three laser sheets perpendicular to the direction of detection. Width, Rayleigh length, focus and thickness of the three light sheets can be set by software. Min. thickness: <4.5 μm @ 488 nm for one individual sheet. Focus range: > ±5 mm from center position of the sample for all usable imaging solutions.

Technical features and specifications:
Objective lens: 2x/0.50
Filters: 488nm, 561nm, 640nm, 785nm
Lasers: 488nm, 561nm, 639nm, 785nm
Cameras: Andor Zyla 4.2 Plus sCMOS Camera
Stage: Motorized XYZ-stage for moving imaging chamber and sample holder
Software: LaVision BioTec ImSpector

Charges:
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Instrument Manufacturer URL: https://www.miltenyibiotec.com/SG-en/products/ultramicroscope-ii.html#gref