Sorter: BD FACSAria II 3-laser Cell Sorter

BD FACSAria II 3-laser Cell Sorter
What is it?
A 9 colour parameters FACS cell sorter that can isolate phenotypically distinct cell populations.

Manufacturer: Becton Dickinson

Model Name: BD FACSARIA II

Applications: Multiplex Cell Analysis and Cell Sorter

Instrument Overview: The BD FACSAria II flow cytometer is a high-speed fixed-alignment benchtop cell sorter with a 3 lasers configuration (Blue, Yellow-green, and Red). With its fixed-optics design and digital electronics, the BD FACSAria III flow cytometer enables multicolor analysis of up to 9 fluorescent and two scatter options at a time. It has the flexibility of performing cell sorting into tubes or plates. For tube sorting, it can sort up to 4 different populations at a time. The sorter can perform single-cell sorting into 96-well plates.

Technical features and specifications:
Software: BD FACSDiva
Sample: Single tube
Lasers: Blue (488nm), Yellow-Green (562nm) and Red (638nm)
Instrument configuration:

Laser	Detector	Fluorochrome	BP Filter	LP Filter	Detection Range
Blue (488-nm)	A	PE-Cy7	780/60	755LP	750nm - 810nm
	B	PerCP, PerCP-Cy5.5, PE-Cy5, 7-AAD, BB700	695/40	685LP	675nm - 715nm
	C	PE-Texas Red, ECD, PI	616/23	610LP	605nm - 627nm
	D	PE	585/42	556LP	573nm - 597nm
	E	FITC, Alexa Fluor 488, GFP, BB515	530/30	502LP	515nm - 545nm
	F	SSC	488/10
	FSC	FSC
Red (638-nm)	A	APC-Cy7	780/60	755LP	750nm - 810nm
	B	Alexa-700	730/45	690LP	715nm - 745nm
	C	APC, Alexa-647	660/20		650nm - 670nm
Violet (405-nm)	A	Qdot 800, BV785	780/60	735LP	750nm - 810nm
	B	Qdot 655, BV650	660/20	630LP	650nm - 670nm
	C	Alexa-605, Qdot-605, eFlour-605, BV605	610/20	600LP	600nm - 620nm
	D	AmCyan, Qdot 525, BV510	510/50	502LP	485nm - 535nm
	E	Pacific Blue, SP (violet), DAPI, eFluor 450, BV421	450/50		425nm - 475nm

Charges:
S$115 per hour

Instrument Manufacturer URL: Becton Dickinson

Detailed Specifications:
https://www.bdbiosciences.com/en-sg/products/instruments/flow-cytometers/research-cell-sorters/bd-facsaria-iii