Agilent: 6224 Time-of-Flight (TOF)

Institute of Molecular and Cell Biology (IMCB)

What is it?
A mass spectrometer that has time-of-flight mass analyzer for mass analysis of various molecules. High sensitivity and speed.

Manufacturer: Agilent Technologies

Model Name: Agilent Accurate-Mass Time-of-Flight 6224

Applications: Mass spectrometry, proteomics

Instrument Overview: The Agilent TOF 6224 delivers exceptional sensitivity, excellent mass accuracy, fast data acquisition, and streamlined qualitative and quantitative analyses.

Technical features and specifications:
Resolution: NA
Mass filter and analyzers: Time-of-flight
Fragmentation: NA
Software: Mass Hunter

Charges:
Depends on project requirement

Instrument Manufacturer URL: https://www.agilent.com/

Detailed Specifications:

Equipment URL

Automated Liquid Handler: Automated Blood Sampler, 2nd gen, Set1

Institute of Molecular and Cell Biology (IMCB)

What is it?
An automated blood sampling machine that allows collection of blood samples in freely-moving animals.

Manufacturer: Instech

Model Name: ABS2

Applications: Automated blood collection

Instrument Overview: Automates the collection of blood samples from laboratory animals including mice, rats and large animals. By eliminating labor-intensive manual withdrawals it can substantially increase the throughput (one technician can run multiple studies simultaneously) of pharmacokinetics research groups, and the ability to collect samples while you are at home sleeping. It can also reduce the stress on animals caused by handling and venipuncture. The sampler withdraws blood from a tethered freely-moving animal according to your programmed schedule, stores the samples in an integrated refrigerated fraction collector, and replaces the withdrawn volumes with IV fluid. You control and monitor sampling schedules, volumes and withdrawal rates for up to 12 animals from a central computer.

Technical features and specifications:
The flexibility to work with a range of species, from mice and rats to large animals
Accurate microsample volumes and accurate time points
Undiluted samples, verified by in-line sensors
Less than five µL of blood wasted per sample, to minimize hemodilution
Fraction collector type: rotary, 10 position;
Software: Metamorph

Charges:
User operated: $50/hour

Instrument Manufacturer URL: https://www.instechlabs.com/products/automated-blood-sampler/abs2

Detailed Specifications:

Automated Liquid Handler: Automated Blood Sampler, 2nd gen, Set2

Institute of Molecular and Cell Biology (IMCB)

What is it?
An automated blood sampling machine that allows collection of blood samples in freely-moving animals.

Manufacturer: Instech

Model Name: ABS2

Applications: Automated blood collection

Instrument Overview: Automates the collection of blood samples from laboratory animals including mice, rats and large animals. By eliminating labor-intensive manual withdrawals it can substantially increase the throughput (one technician can run multiple studies simultaneously) of pharmacokinetics research groups, and the ability to collect samples while you are at home sleeping. It can also reduce the stress on animals caused by handling and venipuncture. The sampler withdraws blood from a tethered freely-moving animal according to your programmed schedule, stores the samples in an integrated refrigerated fraction collector, and replaces the withdrawn volumes with IV fluid. You control and monitor sampling schedules, volumes and withdrawal rates for up to 12 animals from a central computer.

Technical features and specifications:
The flexibility to work with a range of species, from mice and rats to large animals
Accurate microsample volumes and accurate time points
Undiluted samples, verified by in-line sensors
Less than five µL of blood wasted per sample, to minimize hemodilution
Fraction collector type: rotary, 10 position;
Software: Metamorph

Charges:
User operated: $50/hour

Instrument Manufacturer URL: https://www.instechlabs.com/products/automated-blood-sampler/abs2

Detailed Specifications:

Automated Liquid Handler: Automated Blood Sampler, 2nd gen, Set3

Institute of Molecular and Cell Biology (IMCB)

What is it?
An automated blood sampling machine that allows collection of blood samples in freely-moving animals.

Manufacturer: Instech

Model Name: ABS2

Applications: Automated blood collection

Instrument Overview: Automates the collection of blood samples from laboratory animals including mice, rats and large animals. By eliminating labor-intensive manual withdrawals it can substantially increase the throughput (one technician can run multiple studies simultaneously) of pharmacokinetics research groups, and the ability to collect samples while you are at home sleeping. It can also reduce the stress on animals caused by handling and venipuncture. The sampler withdraws blood from a tethered freely-moving animal according to your programmed schedule, stores the samples in an integrated refrigerated fraction collector, and replaces the withdrawn volumes with IV fluid. You control and monitor sampling schedules, volumes and withdrawal rates for up to 12 animals from a central computer.

Technical features and specifications:
The flexibility to work with a range of species, from mice and rats to large animals
Accurate microsample volumes and accurate time points
Undiluted samples, verified by in-line sensors
Less than five µL of blood wasted per sample, to minimize hemodilution
Fraction collector type: rotary, 10 position;
Software: Metamorph

Charges:
User operated: $50/hour

Instrument Manufacturer URL: https://www.instechlabs.com/products/automated-blood-sampler/abs2

Detailed Specifications:

Automated Liquid Handler: Automated Blood Sampler, 2nd gen, Set4

Institute of Molecular and Cell Biology (IMCB)

What is it?
An automated blood sampling machine that allows collection of blood samples in freely-moving animals.

Manufacturer: Instech

Model Name: ABS2

Applications: Automated blood collection

Instrument Overview: Automates the collection of blood samples from laboratory animals including mice, rats and large animals. By eliminating labor-intensive manual withdrawals it can substantially increase the throughput (one technician can run multiple studies simultaneously) of pharmacokinetics research groups, and the ability to collect samples while you are at home sleeping. It can also reduce the stress on animals caused by handling and venipuncture. The sampler withdraws blood from a tethered freely-moving animal according to your programmed schedule, stores the samples in an integrated refrigerated fraction collector, and replaces the withdrawn volumes with IV fluid. You control and monitor sampling schedules, volumes and withdrawal rates for up to 12 animals from a central computer.

Technical features and specifications:
The flexibility to work with a range of species, from mice and rats to large animals
Accurate microsample volumes and accurate time points
Undiluted samples, verified by in-line sensors
Less than five µL of blood wasted per sample, to minimize hemodilution
Fraction collector type: rotary, 10 position;
Software: Metamorph

Charges:
User operated: $50/hour

Instrument Manufacturer URL: https://www.instechlabs.com/products/automated-blood-sampler/abs2

Detailed Specifications:

Automated Liquid Handler: Automated Blood Sampler, 2nd gen, Set5

Institute of Molecular and Cell Biology (IMCB)

What is it?
An automated blood sampling machine that allows collection of blood samples in freely-moving animals.

Manufacturer: Instech

Model Name: ABS2

Applications: Automated blood collection

Instrument Overview: Automates the collection of blood samples from laboratory animals including mice, rats and large animals. By eliminating labor-intensive manual withdrawals it can substantially increase the throughput (one technician can run multiple studies simultaneously) of pharmacokinetics research groups, and the ability to collect samples while you are at home sleeping. It can also reduce the stress on animals caused by handling and venipuncture. The sampler withdraws blood from a tethered freely-moving animal according to your programmed schedule, stores the samples in an integrated refrigerated fraction collector, and replaces the withdrawn volumes with IV fluid. You control and monitor sampling schedules, volumes and withdrawal rates for up to 12 animals from a central computer.

Technical features and specifications:
The flexibility to work with a range of species, from mice and rats to large animals
Accurate microsample volumes and accurate time points
Undiluted samples, verified by in-line sensors
Less than five µL of blood wasted per sample, to minimize hemodilution
Fraction collector type: rotary, 10 position;
Software: Metamorph

Charges:
User operated: $50/hour

Instrument Manufacturer URL: https://www.instechlabs.com/products/automated-blood-sampler/abs2

Detailed Specifications:

Automated Liquid Handler: Automated Microplate Spotter/Dispenser Unit

Institute of Molecular and Cell Biology (IMCB)

Automated Dispenser Unit

Automated Liquid Handler: Bravo-V11-Benchcell

Institute of Molecular and Cell Biology (IMCB)

Agilent Bravo V11 BenchCell Plate sealing Barcode printing liquid handling solution

Automated Liquid Handler: C.WASH Centrifugal low g multiwell plate washer

Institute of Molecular and Cell Biology (IMCB)

C.WASH Centrifugal low g muttiwell plate washer What is it? An unconventional plate washer who does not use pin to remove liquid but low g centrifugation of down to ~20g. System can also dispense washing buffer after washing. Works with 96/384 fo dispensing. Works with any plate for washing for 96/384/1536 plates. Manufacturer: Cytena Model Name: C.WASH Applications: ELISA, cell assays, plate based assay Detailed Specifications: https://www.cytena.com/products/microplate-washers-and-dispensers/c-wash/

Equipment URL

Automated Liquid Handler: Formulatrix NT8 Nanoliter-volume liquid handler

Institute of Molecular and Cell Biology (IMCB)

AMPLE HTML code for ARIN PPMS Equipment Description Formulatrix NT8 Nanoliter-volume liquid handler w/LCP What is it?
A crystallisation robot/ drop setter with drop dispensing capability.

Manufacturer: Formulatrix

Model Name: NT8

Applications: Protein Crystallography

Instrument Overview: The NT8® - Drop Setter is a nanoliter-volume liquid handler with drop dispensing capability. The 8-tip head aspirates and dispenses drops from 10 nL to 1.5 μL.

Technical features and specifications:
High Volume Pipette Tips: The high-volume tips used for the Plate Copy Option are made by Rainin™.
Operating Temperature: Room Temperature
Humidity: 0 to 80% relative humidity

Charges:
User operated: $200/day, Staff operated: $400/day

Instrument Manufacturer URL: https://formulatrix.com/protein-crystallization-systems/nt8-drop-setter/

Detailed Specifications:
N.A.

Automated Liquid Handler: I.DOT S non contact nano liter dispenser

Institute of Molecular and Cell Biology (IMCB)

I.DOT S nanoliter dispenser. 96 to 96/384 well plate. What is it? A non contact dispenser who can dispense in an upright manner nanoliter drops from a 96 well source plate to 384/96 well target plate and checks/validate proper dispense. The system requires to use/purchase S.100 96 well plate for loading source compounds, and has a minimum volume of 5 to 10ul to load in for proper dispense to target well. Manufacturer: Cytena Model Name: I.DOT S Applications: Compound screen, cell assays, Miniaturization of reaction, array dispensing Detailed Specifications: https://www.cytena.com/products/liquid-handlers/i-dot/

Equipment URL

Automated Liquid Handler: Liquid Handling System 96 Channel Biomek NX

Institute of Molecular and Cell Biology (IMCB)

What is it?
A liquid handling system.

Manufacturer: Beckman Coulter

Model Name: Biomek NX

Applications: Liquid handling

Instrument Overview: Liquid Handler

Technical features and specifications:







Charges:


Instrument Manufacturer URL: NA

Detailed Specifications:

Automated Liquid Handler: Liquid Handling System Phoenix

Institute of Molecular and Cell Biology (IMCB)

Liquid Handling System Phoenix What is it?
A dispensing system used for setting up 96-well crystallisation plates

Manufacturer: Art Robbins Instruments

Model Name: Phoenix

Applications: Protein crystallography

Instrument Overview: The Phoenix is the ideal dispensing system for working with organic solvents. The reagents contact the syringe head and are only exposed to glass, stainless steel and a fluoropolymer making the head compatible with all the commonly used reagents. The deck of the Phoenix has 6 plate positions and 3 wash positions. The 1mL syringes can access deep well blocks and tube racks. The all metal construction features hard anodized decks. Easy to use drag and drop software makes it quick to develop new protocols or modify existing ones. The Phoenix comes with 1 nano dispenser.

Technical features and specifications:
Dispense volumes: 96 Head: 1.0 µL to 1000 µLwith 1 mL syringe head, other sizes available
Size: Base: 29(73.6)h x 25(63.5)w x 27(68.6)d in(cm)
Weight: 142 lbs, 64.4 Kg
Electrical: 120/240 Vac, 3 A, 50/60 Hz
Software Requirement: Windows 7 and .NET

Charges:
User operated: $200/day, Staff operated: $400/day

Instrument Manufacturer URL:https://www.artrobbins.com/phoenix

Detailed Specifications:
N.A.

Automated Staining & Scanning System: COMET 1.0

Institute of Molecular and Cell Biology (IMCB)

What is it?
High-throughput Automated Slide Stainer and Scanner

Manufacturer: Lunaphore Technologies

Model Name: COMET 1.0

Applications: Multiplex sequential immunofluorescence

Instrument Overview: The only high-throughput and hyperplex platform that allows scalability and reproducibility without the need for conjugation.

Technical features and specifications:
Plex level: Virtually unlimited plex level capability of technology. Performs 40 markers per automated run Slide capacity: 4 Automation level: Fully automated: staining, image acquisition, image preprocessing Maximum imaging area and staining area: Max imaging area: 12.5mm x 12.5mm / 0.49in x 0.49in, Staining area: 21mm x 21mm / 0.82in x 0.82in Microscope magnification and numerical aperture: 20X / 0.7NA Image resolution: 0.28um/pixel

Charges:
NA

Instrument Manufacturer URL: NA

Detailed Specifications:
NA

Automated Staining System: BOND-MAX

Institute of Molecular and Cell Biology (IMCB)

What is it?
Leica Fully Automated IHC and ISH Slide Stainer (for IVD use only)

Manufacturer: Leica Biosystems

Model Name: BOND-MAX

Applications: IHC staining, ISH staining

Instrument Overview: Delivers high-quality IHC and ISH slides staining with precise automation, by combining Covertile technology and Novocastra reagents.

Technical features and specifications:
Slide capacity: 30 Reagent container capacity: 7mL and 30mL Number of reagent containers: 36 Bulk reagent container capacity: 1L or 2L Hazardous waste container capacity: 2L External bulk waste container capacity: 9L

Charges:
NA

Instrument Manufacturer URL: https://www.leicabiosystems.com/en-sg/ihc-ish/ihc-ish-instruments/bond-max-fully-automated-ihc-and-ish/

Detailed Specifications:
https://www.leicabiosystems.com/en-sg/ihc-ish/ihc-ish-instruments/bond-max-fully-automated-ihc-and-ish/

Bruker: ESI TIMS-Time-of-Flight SCP

Institute of Molecular and Cell Biology (IMCB)

What is it?
A cutting-edge mass spectrometer that combines a MALDI or ESI source, ion mobility, and time-of-flight mass analyzer for mass analysis of various molecules. High sensitivity and speed.

Manufacturer: Bruker

Model Name: MALDI/ESI TIMS-TOF

Applications: Mass spectrometry, proteomics, metabolomics

Instrument Overview: The TIMS-TOF Mass Spectrometer enables scientists analyzing the most challenging low-abundance, high-complexity samples to identify more compounds faster, quantify more accurately and elucidate structures more thoroughly.

Technical features and specifications:
Resolution: NA
Mass filter and analyzers: Ion mobility and time-of-flight
Fragmentation: Collision-induced dissociation (CID)
Software: Hystar

Charges:
Depends on project requirement

Instrument Manufacturer URL: NA

Detailed Specifications:

Bruker: MALDI/ESI TIMS-Time-of-Flight

Institute of Molecular and Cell Biology (IMCB)

What is it?
A cutting-edge mass spectrometer that combines a MALDI or ESI source, ion mobility, and time-of-flight mass analyzer for mass analysis of various molecules. High sensitivity and speed.

Manufacturer: Bruker

Model Name: MALDI/ESI TIMS-TOF

Applications: Mass spectrometry, proteomics, metabolomics

Instrument Overview: The TIMS-TOF Mass Spectrometer enables scientists analyzing the most challenging low-abundance, high-complexity samples to identify more compounds faster, quantify more accurately and elucidate structures more thoroughly.

Technical features and specifications:
Resolution: NA
Mass filter and analyzers: Ion mobility and time-of-flight
Fragmentation: Collision-induced dissociation (CID)
Software: Hystar

Charges:
Depends on project requirement

Instrument Manufacturer URL: NA

Detailed Specifications:

Capillary-based automated Western: Simple Western system (Wes)

Institute of Molecular and Cell Biology (IMCB)

What is it?
Simple Western is a next-generation hands-free capillary immunoassay platform combining protein separation with sensitive chemiluminescence and fluorescence immunodetection using conventional primary and conjugated secondary antibodies..

Manufacturer: ProteinSimple

Model Name: Wes

Applications: Western

Instrument Overview: Simple Westerns take place in a capillary. Wes automates all steps of the process for you including sample loading, protein separation, immunoprobing or labeling, washing, detection and data analysis. Up to 25 samples can be processed in a single runWes performs Simple Western size-based assays: Immunoassay and Total Protein assay. All you have to do is pipette your samples and immunoreagents into Wes's pre-filled plate, load the capillary cartridge and press start. br>
Technical features and specifications:
Objective lens: NA
Filters: NA
Light sources: Transmitted (LG-PS2 halogen), Fluorescence (Mercury burner)
Cameras: NA
Stage: NA
Software: Compass for Simple Western

Charges:
User operated: $10.30/hour, Staff operated: $67/hour

Instrument Manufacturer URL: NA

Detailed Specifications:
https://www.bio-techne.com/instruments/simple-western

Cell Analyzer: ACEA Biosciences MP System - Xcelligence

Institute of Molecular and Cell Biology (IMCB)

What is it?
Real-Time Cell Analysis (RTCA) MP instrument that measures cell index with cellular impedance

Manufacturer: Agilent

Model Name: xCELLigence RTCA MP

Applications: Immune Cell Killing Assays

Instrument Overview: The Agilent xCELLigence Real-Time Cell Analysis (RTCA) MP instrument uses label-free cellular impedance to continuously monitor cell health, behavior, and function with high accuracy, sensitivity, and reproducibility

Technical features and specifications:
MP system
Software: RTCA Pro Basic

Charges:
User operated: $40/hr

Instrument Manufacturer URL: https://www.agilent.com/en/product/cell-analysis/real-time-cell-analysis/rtca-analyzers/xcelligence-rtca-mp-multiple-plates-741230#howitworks

Cell Analyzer: BD LSRFortessa Cell Analyzer with BD High

Institute of Molecular and Cell Biology (IMCB)

BD Cell Analyzer

Cell Sorter: MACSQuant Tyto Cell Sorter

Institute of Molecular and Cell Biology (IMCB)

What is it?
Cell Sorter

Manufacturer: Miltenyi Biotec

Model Name: MACSQuant Tyto Cell Sorter

Applications: Cell Sorting

Instrument Overview: Cell Sorter

Technical features and specifications:
Lasers: 405nm, 488nm, 638nm
Filters: 450/45, 525/50, 525/50, 585/40, 655-730, 750, 655-730, 750
Sample holder: MACSQuant Tyto Cartridge
Software: Tyto Breeze

Charges:
Users to use their own cartridges. ASTAR 180/hr, Public/Academic 195/hr

Instrument Manufacturer URL: https://www.miltenyibiotec.com/SG-en/products/macsquant-tyto.html

Detailed Specifications:
https://www.miltenyibiotec.com/SG-en/products/macsquant-tyto.html

Cell processing and production: CellXpressAI

Institute of Molecular and Cell Biology (IMCB)

temporary

Cell processing and production: Seahorse O2/CO2 Flux Analyzer

Institute of Molecular and Cell Biology (IMCB)

What is it?
Agilent Seahorse XFe24 Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 24-well plate format.

Manufacturer: Agilent

Model Name: Seahorse XFe24

Applications: Oxygen consumption rate, extracellular acidification rate, cell metabolic function in cell culture

Instrument Overview: OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate, and together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.

Technical features and specifications:
24-well microplate format
Reports real-time metabolic rates in minutes
Four-port injection system with automated mixing function
Precision-controlled heating tray to maintain temperatures at 16-42 degree
Sample requirements: 10000-1000000 cells/well
Software: wave controller

Charges:
User operated: $100/hour (ASTAR users), $200/hour (external users), Staff operated: NA. Please contact the lab before booking.

Instrument Manufacturer URL: https://www.agilent.com/en/product/cell-analysis/real-time-cell-metabolic-analysis/xf-analyzers/seahorse-xfe24-analyzer-740878

Detailed Specifications:
https://www.agilent.com/en/product/cell-analysis/real-time-cell-metabolic-analysis/xf-analyzers/seahorse-xfe24-analyzer-740878

Cell processing and production: VITROCELL® Cloud Alpha MOVE

Institute of Molecular and Cell Biology (IMCB)

https://www.vitrocell.com/inhalation-toxicology/exposure-systems/vitrocell-cloud-system/vitrocell-cloud-alpha-move/

Circular Dichroism Spectropolarimeter: Jasco J-810 Circular Dichroism Spectropolarimeter

Institute of Molecular and Cell Biology (IMCB)

Jasco J-810 Circular Dichroism Spectropolarimeter What is it?
A hybrid instrument consisting of a variable wavelength polarimeter and absorption spectrophotometer

Manufacturer: Jasco

Model Name: J-810

Applications: Protein conformational studies, Protein folding studies, DNA/RNA interactions, Enzyme kinetics, Purity testing of optically active substances

Instrument Overview: The Jasco J-810 Spectropolarimeter (163-900 nm) is a hybrid instrument consisting of a variable wavelength polarimeter and absorption spectrophotometer. It is designed to allow secondary structure determination by circular dichroism; specifically measuring the left-and right-handed circularly polarized light of optically active molecules. It is equipped with a computer-controlled Peltier device (3-90 °C) and a two-syringe titrator to assess thermal/chemical stabilities of proteins/ polypeptides or to monitor ligand binding and is controlled by Jasco’s Spectra Manager software.

Technical features and specifications:
Light source: 150W air cooled Xe lamp or 450W water cooled Xe lamp (option)
Detector: Head-on photomultiplier tube
Modulator: Piezoelastic modulator
Wavelength accuracy: ±0.2 nm (at 163 to 180 nm) ±0.1 nm (at 180 to 250 nm) ±0.3 nm (at 250 to 500 nm) ±0.8 nm (at 500 to 800 nm) ±2.0 nm (at 800 to 1100 nm)
Spectral Bandwidth: 0.01 to 15 nm
Slit width: 1 to 3000 μm
Response: 0.5 msec to 32 sec
Scanning system: Continuous scan Step scan (Fixed response and auto response system)
Scanning speeds: 1 to 10000 nm/min (continuous scan) Data interval: 0.025 to 10 nm (continuous scan) 0.1 to 100 nm (step scan) 0.5 msec to 60 min (time change)
CD full scale: ±10, 200, 2000 mdeg
CD resolution: 0.0005 mdeg (at ±10 mdeg full scale) 0.01 mdeg ( at ±200 mdeg full scale) 0.1 mdeg ( at ±2000 mdeg full scale)
Stray light: Less than 0.0003% (200 nm)
RMS noise: 185 nm:0.050 mdeg (150W Xe lamp) 200 nm:0.040 mdeg (150W Xe lamp) 500 nm:0.035 mdeg (150W Xe lamp) (Spectal bandwidth 1 nm, response 16 sec)
Baseline Stability: ±0.03 mdeg/hr (Spectal bandwidth 1 nm, response 32 sec, wavelength 290 nm)
External input terminal: Two channels (input range: -1 to 1 V)
Shutter: Opens and closes in front of sample
Sample chamber: Small size (S type) and large size (L type) are available Small size chamber dimensions: 140 (W) x 300 (D) x 130 (H) mm Large size chamber dimensions: 305 (W) x 420 (D) x 270 (H) mm
Nitrogen gas purge: Atmosphetr in the light source unit, monochromator unit, and sample chamber is displaced using dry nitrogen gas.

Charges:
User operated: $30/h, Staff operated: $50/h

Instrument Manufacturer URL:https://jascoinc.com/products/spectroscopy/circular-dichroism/

Detailed Specifications:
N.A.

Clinical Analyser: Automated Haematology Analyser

Institute of Molecular and Cell Biology (IMCB)

What is it?
Automated Haematology Analyser.

Manufacturer: Sysmex

Model Name: XN10

Applications: Automated Haematology Analyser

Charges: FBC + Diff Count - $35/sample
FBC + Diff Count +Ret - $40/sample

Dynamic Nuclear Polarizer: SpinAligner

Institute of Molecular and Cell Biology (IMCB)

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Electrophysiology: Electrophysiology Whole Patch Clamp System

Institute of Molecular and Cell Biology (IMCB)

What is it?
Complete electrophysiology system for patch clamp recording.

Manufacturer: Scientifica, Oimaging, CoolLED, AM-Systems, Axon Instruments

Model Name: Scientifica SliceScope Pro 2000, Qimaging optiMOS™ Camera, CoolLED pE-4000, A-M Systems Model 2100, Axon Instruments MultiClamp 700B, Axon Digidata 1550B

Applications: Electrophysiology

Instrument Overview: The system includes the following: 1. Scientifica SliceScope system that includes a slimline, fully motorised microscope, Scientifica PatchStar Micromanipulators, Scientifica Motorised XY stage and Scientifica SlicePlatform 2. Scientific OptiMOS™ CMOS camera 3. Isolated Pulse Stimulator for physiological stimulation protocols 4. Computer-controlled microelectrode current and voltage clamp amplifier for electrophysiology and electrochemistry 5. An analog-to-digital signal converter for electrophysiological data acquisition.

Technical features and specifications:
Objective lens: Olympus Plan N 4x/0.1, Olympus LUMPlanFL N 40x/0.80 W
Filters: B/IR, GFP, TXRED
Light sources Transmitted (Olympus TH4-200), Fluorescence (CoolLED pE-4000)
Cameras: Qimaging optiMOS™ Camera
Stage: Motorised
Software: Clampex 10.7.0.3, Clampfit 10.7.0.3, MultiClamp 700B Commander, AxoGraph, MMStudio Version 2.0.0-beta 3, LinLab 2

Charges:
NA

Instrument Manufacturer URL: NA

Detailed Specifications:

Electrophysiology: Integrated electrophysiology system: Patch Clamp

Institute of Molecular and Cell Biology (IMCB)

What is it?
Complete electrophysiology system for patch clamp recording.

Manufacturer: Axon Instruments/HEKA Elektronik

Model Name: Axon Instruments MultiClamp 700B, Axon Digidata 1550B/ HEKA EPC10 USB

Applications: Electrophysiology

Instrument Overview: The system includes the following: 1. Nikon microscope with manual XY stage and Micromanipulator 2. Scientific CMOS camera 3. Isolated Pulse Stimulator for physiological stimulation protocols 4. Computer-controlled microelectrode current and voltage clamp amplifier for electrophysiology and electrochemistry 5. An analog-to-digital signal converter for electrophysiological data acquisition.

Technical features and specifications:
Objective lens: 1x, 40x (water immersion)
Filters: GFP, TRITC
Light sources: Transmitted, Fluorescence (Mercury lamp)
Stage: Manual
Software: Clampex 10.7.0.3, Clampfit 10.7.0.3, MultiClamp 700B Commander/PatchMaster

Charges:
User operated: $100/hour (ASTAR users only), Staff operated: NA. Please contact the lab before booking.

Instrument Manufacturer URL: NA

Detailed Specifications:
https://www.heka.com/products/products_main.html#physiol_epc10single

Electrophysiology: Multi Electrode Array w/ Multiwell, Singlewell

Institute of Molecular and Cell Biology (IMCB)

Multi Electrode Array w/ Multiwell, Singlewell & Analysis Software What is it?
The MEA device captures functional behaviour (i.e. electrophysiological activity) of any electroactive cells, such as neurons or cardiomyocytes. It allows extracellular recording of action potentials, otherwise known as field potentials.

Manufacturer: Multi Channel Systems

Model Name: Multiwell-MEA-System, MEA2100-Lite-System

Applications: Multi Electrode Array, Electrophysiology

Instrument Overview: Featuring a 24- or 96-well plate format, the Multiwell-MEA system is the perfect tool for medium to high throughput in vitro electrophysiology. The system includes high-quality, low-noise amplifiers, freely programmable stimulators and the data is sampled at up to 50 kHz per channel (simultaneously on all channels). The MEA2100-Lite-System is a compact and portable stand-alone system that uses a headstage that will fit on inverted and upright microscopes.

Technical features and specifications:
USB 3.0 interface to data acquisition computer
Sampling rate: 50 kHz per channel
Headstage functions: 288 channel amplifiers, analog/digital conversion, integrated heating, integrated stimulator, current and voltage stimulation, selectable via software
Well plate formats: 24-well plates with 12 electrodes each and 96-well plates with 12 or 3 electrodes in each well
Data acquisition and analysis softwares: Multiwell-Screen and Multiwell-Analyzer
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Charges:
NA

Instrument Manufacturer URL: NA

Detailed Specifications:
https://www.multichannelsystems.com/products/multiwell-mea-system

Electrophysiology: Multielectrode Array system, Maestro Pro

Institute of Molecular and Cell Biology (IMCB)

Multielectrode Array system, Maestro Pro

Equipment URL

FPLC: AKTA Pure 25 L (only used for RNA work)

Institute of Molecular and Cell Biology (IMCB)

What is it?
A chromatography system for purifying proteins.

Manufacturer: Cytiva

Model Name: AKTA Pure 25 L

Applications: Protein purification

Instrument Overview: Flexible chromatography system for purification of proteins.

Technical features and specifications:
Depth: 470mm
Flow cell path length: 2mm
Flow rate: 0.001 to 25 mL/min (normal range), 0.01 to 50 mL/min (column packing flow)
Height: 629mm
Operating pressure max: 20 MPa
Software: UNICORN

Charges:
User operated: $60/hour, Staff operated: $150/hour

Instrument Manufacturer URL: NA

Detailed Specifications:
http://tinyurl.com/ydzr2782

FPLC: AKTA Pure 25 M1-FPLC System (RNA work only)

Institute of Molecular and Cell Biology (IMCB)

AKTA Pure 25 M1-Fast Protein Liquid Chromatography(FPLC) System for RNA work only What is it?
A FPLC System for purification of proteins, peptides, and nucleic acids

Manufacturer: Cytiva

Model Name: AKTA pure 25 M1

Applications: Protein/peptide/nucleic acid purification

Instrument Overview: Flexible and intuitive chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram to gram levels of target product.

Technical features and specifications:
Automatic buffer preparation: No
Autosampler injection: No
Buffer selection: 2A + 2B inlets in one valve
Compliance: The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva.
Flow cell path length: 2 mm (optional 0.5 mm and 10 mm)
Flow rate: 0.001 to 25 mL/min (normal range), 0.01 to 50 mL/min (column packing flow)
Multiple wavelength detection: 280nm and 260nm
Pump type: Piston pump, metering type
Relative humidity: 20% to 95% (noncondensing)
Tubing i.d. (flow path): 0.5 mm
UV wavelength: 190 to 700 nm
Viscosity: 0.35 to 10.0 cP (system pump)

Charges:
User operated: $400/day, Staff operated: $800/day

Instrument Manufacturer URL: https://www.cytivalifesciences.com/en/sg/shop/chromatography/chromatography-systems/akta-pure-p-05844#tech-spec-table

Detailed Specifications:
N.A.a>

FPLC: AKTA pure 25 M1 (P2ERL)

Institute of Molecular and Cell Biology (IMCB)

What is it?
AKTA™ pure chromatography system is a highly versatile, modular system with a number of design features to facilitate reliable purification.

Manufacturer: Cytiva

Model Name: AKTA pure 25 M

Applications: Flexible and intuitive chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram to gram levels of target product.

Instrument Overview: AKTA pure chromatography system allows for quick and easy customization thanks to the modular design of both the hardware and software. Choose from AKTA pure 25 with a flow rate up to 25 mL/min or AKTA pure 150 with a flow rate up to 150 mL/min and combine with more than 20 hardware options.

Technical features and specifications:
Application supported: Affinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R
Flow rate: 0.001 to 25 mL/min (normal range), 0.01 to 50 mL/min (column packing flow)
Multiple wavelength detection: Up to 3 wavelengths
UV wavelength: 190-700 nM
Flow cell path length: 2 mm (optional 0.5 mm and 10 mm)
Autosampler injection: No
pH monitoring: 0-14 (optional)
Multistep purification: Yes

Charges: NA

Instrument Manufacturer URL:
https://cdn.cytivalifesciences.com/dmm3bwsv3/AssetStream.aspx?mediaformatid=10061&destinationid=10016&assetid=16276
Detailed Specifications: NA

FPLC: AKTAxpress - MODULE 1 AMERSHAM BIOSCIENCES

Institute of Molecular and Cell Biology (IMCB)

Protein purification system

FPLC: FPLC System- AKTA pure 25 M1

Institute of Molecular and Cell Biology (IMCB)

Fast Protein Liquid Chromatography (FPLC) System -AKTA pure 25 M1 What is it?
A FPLC System for purification of proteins, peptides, and nucleic acids

Manufacturer: Cytiva

Model Name: AKTA pure 25 M1

Applications: Protein/peptide/nucleic acid purification

Instrument Overview: Flexible and intuitive chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram to gram levels of target product.

Technical features and specifications:
Automatic buffer preparation: No
Autosampler injection: No
Buffer selection: 2A + 2B inlets in one valve
Compliance: The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva.
Flow cell path length: 2 mm (optional 0.5 mm and 10 mm)
Flow rate: 0.001 to 25 mL/min (normal range), 0.01 to 50 mL/min (column packing flow)
Multiple wavelength detection: 280nm and 260nm
Pump type: Piston pump, metering type
Relative humidity: 20% to 95% (noncondensing)
Tubing i.d. (flow path): 0.5 mm
UV wavelength: 190 to 700 nm
Viscosity: 0.35 to 10.0 cP (system pump)

Charges:
User operated: $300/day, Staff operated: $500/day

Instrument Manufacturer URL: https://www.cytivalifesciences.com/en/sg/shop/chromatography/chromatography-systems/akta-pure-p-05844#tech-spec-table

Detailed Specifications:
N.A.a>

Fish-tank Rack: System 3 - 1

Institute of Molecular and Cell Biology (IMCB)

TBA

Flow: BD 4L FACS Aria Cell Sorter

Institute of Molecular and Cell Biology (IMCB)

What is it?
Cell Sorter.

Manufacturer: BD

Model Name: FACS Aria II

Applications: Cell Sorting/Analysis

Instrument Overview: 4 laser cell sorter

Technical features and specifications:







Charges:


Instrument Manufacturer URL: NA

Detailed Specifications:

Flow: BD A3 Analyzer

Institute of Molecular and Cell Biology (IMCB)

What is it?
A cell analyzer.

Manufacturer: BD

Model Name: A3

Applications: Cell Analysis

Instrument Overview:
Technical features and specifications:

Charges:


Instrument Manufacturer URL: NA

Detailed Specifications:

Flow: BD Celesta

Institute of Molecular and Cell Biology (IMCB)

Celesta What is it?
A Flow Cytometry analyzerbr>
Manufacturer: BD

Model Name: Celesta

Applications: Flow Cytometry Analysis

Instrument Overview: 4L Cell Analyzerbr>
Technical features and specifications:
5B/3R/6V/2UV

Charges:
User operated: $110/hour, Staff operated: $130/hour

Instrument Manufacturer URL: NA

Detailed Specifications:

Flow: BD LSR Fortessa X20

Institute of Molecular and Cell Biology (IMCB)

LSR Fortessa X20 What is it?
A Flow Cytometry analyzerbr>
Manufacturer: BD

Model Name: LSR Fortessa X20

Applications: Flow Cytometry Analysis

Instrument Overview: 4L Cell Analyzerbr>
Technical features and specifications:
5B/3R/6V/2UV

Charges:
User operated: $110/hour, Staff operated: $130/hour

Instrument Manufacturer URL: NA

Detailed Specifications:

Flow: BD S6 Sorter

Institute of Molecular and Cell Biology (IMCB)

What is it?
A spectral sorter.

Manufacturer: BD

Model Name: S6

Applications: Cell Sorting

Instrument Overview:
Technical features and specifications:

Charges:


Instrument Manufacturer URL: NA

Detailed Specifications:

Flow: BD Symphony A5

Institute of Molecular and Cell Biology (IMCB)

What is it?
Cell Analyzer.

Manufacturer: BD

Model Name: FACS Symphony A5.2

Applications: Cell Analysis

Instrument Overview: 5 laser cell spectral cell analyzer

Technical features and specifications:







Charges:


Instrument Manufacturer URL: NA

Detailed Specifications:

Flow: BD Symphony A5.2

Institute of Molecular and Cell Biology (IMCB)

What is it?
Cell Analyzer.

Manufacturer: BD

Model Name: FACS Symphony A5.2

Applications: Cell Analysis

Instrument Overview: 5 laser cell spectral cell analyzer

Technical features and specifications:







Charges:


Instrument Manufacturer URL: NA

Detailed Specifications:

Flow: Cytek Aurora 3L

Institute of Molecular and Cell Biology (IMCB)

What is it?
Cell Analyzer.

Manufacturer: Cytek

Model Name: Aurora

Applications: Cell Analysis

Instrument Overview: 5 laser cell spectral cell analyzer

Technical features and specifications:







Charges:


Instrument Manufacturer URL: NA

Detailed Specifications:

Flow: Cytek Aurora Demo Unit 4L

Institute of Molecular and Cell Biology (IMCB)

What is it?
Cell Analyzer.

Manufacturer: Cytek

Model Name: Aurora

Applications: Cell Analysis

Instrument Overview: 5 laser cell spectral cell analyzer

Technical features and specifications:







Charges:


Instrument Manufacturer URL: NA

Detailed Specifications:

Flow: CytoFLEX LX Flow Cytometer

Institute of Molecular and Cell Biology (IMCB)

What is it?
Flow cytometer

Manufacturer: Beckman Coulter

Model Name: CytoFLEX LX Flow Cytometer

Applications: Flow Cytometry

Instrument Overview: Flow Cytometry

Technical features and specifications:
Lasers: 405nm, 488nm, 561nm, 638nm
Filters: 450/45 (Pacific Blue), 525/40 (Krome Orange), 610/20 (BV610), 660/20 (BV660), 763/43 (V763), 525/40 (FITC), 610/20 (ECD), 690/50 (PC5.5), 585/42 (PE), 610/20 (ECD/mCherry), 675/30 (PC5), 710/50 (PC5.5), 660/10 (APC), 712/25 (APC A700), 780/60 (APC A750)
Sample holder: 96well plate, FACS tubes
Software: CyteExpert

Charges:
$60-66/h

Instrument Manufacturer URL: https://www.beckman.com/flow-cytometry/research-flow-cytometers/cytoflex-lx

Detailed Specifications:
https://tinyurl.com/Cytoflex

Flow: Guava EasyCyte 5HT

Institute of Molecular and Cell Biology (IMCB)

What is it?
Flow cytometer

Manufacturer: Luminex

Model Name: Guava EasyCyte 5HT

Applications: Cell counting

Instrument Overview: Cell counting via flow capillary method

Technical features and specifications:
Sample holder: 96well plate (Specific plate required)
Software: InCyte

Charges:
To be updated

Instrument Manufacturer URL: https://www.luminexcorp.com/guava-easycyte-flow-cytometers/#overview

Detailed Specifications:
https://tinyurl.com/Guava

IHC AUTOSTAINER: LEICA BOND-III

Institute of Molecular and Cell Biology (IMCB)

What is it?
IHC Autostainer for Brightfield IHC staining of paraffin embedded sections on microscope slides.

Manufacturer: Leica

Model Name: Bond-III

Applications: Automated IHC Autostainer

Charges: $38/slide

IHC AUTOSTAINER: ROCHE DISCOVERY ULTRA

Institute of Molecular and Cell Biology (IMCB)

What is it?
IHC Autostainer for Brightfield IHC staining of paraffin embedded sections on microscope slides.

Manufacturer: Leica

Model Name: Bond-III

Applications: Automated IHC Autostainer

Charges: $38/slide