Analytics: Orbitrap Fusion with ETD
Analytics: Orbitrap Fusion with ETD
Bioprocessing Technology Institute (BTI)
What is it?
The Orbitrap Fusion tribrid is a high resolution and high sensitivity mass spectrometer that utilised the Orbitrap detector and ion trap detector for glycoproteomics analysis and relative quantitation with and without tandem mass tags. It is coupled to a Thermo nanoLC that provides high sensitivity analysis using small amounts of samples.
Manufacturer: Thermo Fisher Scientific
Model Name: Orbitrap Fusion Tribrid with ETD
Applications: Glycoproteomics analysis (intact and non-intact) for N- and O-glycopeptides
Instrument Overview: The mass spectrometer has an Orbitrap detector and ion trap detector that allows for high resolution analysis (up to 500,000) and relative quantitation with and without tandem mass tags (TMT). It has five types of multiple dissociation techniques, HCD, CID ETD, EthcD and EtciD and provides flexibility for combination of different fragmentation at any stage of MSn. It can switch between 3 ionization sources – ESI, nanospray flex and Easy spray nano source. It is coupled to a Thermo nanoLC that provides high sensitivity analysis using small amounts of samples at microlitre injections.
Technical features and specifications:
Mass range: m/z 50 to 6000
Resolution: 7,500 to 500,000
Mass analyzer: Quadrupole, ion-routing multipole
Mass detector: Orbitrap and Ion trap
Fragmentation mode: HCD, CID, ETD, EthcD, EtciD
Acquisition mode: Full scan MS, MSn, parallel reaction monitoring (PRM), selected ion monitoring (SIR), Data dependent acquisition (DDA), Data independent acquisition (DIA), Automated Synchronous Precursor Selection (SPS) for MS3
Software: Xcalibur and Orbitrap fusion Tune (instrument control), Proteome discoverer, Byonic, BiopharmaFinder (data processing)
Charges:Dependent on project requirement
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Instrument Manufacturer URL: https://www.thermofisher.com/order/catalog/product/IQLAAEGAAPFADBMBCX
Detailed Specifications:
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